Packages - Puxano

PUXANO Service Packages

Standardized services with defined timelines and results

Explore our streamlined stand-alone Service Packages, crafted for efficient, high-throughput protein analysis. These packages represent a simplified step from our Design-to-Structure and Gene-to-Structure workflows, offering standardized, quick-to-initiate services. With just a few details, you can get started easily, knowing that you’ll receive your results promptly without any unnecessary steps. It’s as simple as selecting your package, submitting your samples, and receiving reliable results—all in a short timeframe.

Our service packages

Protein Analysis

Comprehensive bioinformatics and meta-analysis of the protein target, including biological, patent, and mechanism of action (MoA) information.

Protein Analysis

Input: Protein target ID and purpose
Process: Utilizing our proprietary ProCondor platform, we analyze the protein target at several levels. This includes biological information, patent searches, literature research related to protein production, as well as biophysical and structural data. Additionally, we conduct a competitive scan around the MoA and similar solutions.
Output: A detailed description of the protein target across multiple dimensions.
Timeline: 1 week per protein sample

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Protein Design

Protein design

Input: Protein target ID and purpose
Process: Based on the protein analysis information, we define various protein design and engineering strategies. These strategies are developed using AI-assisted algorithms and structure-guided protein design techniques, followed by a thorough in silico selection process based on different success prediction scores. The defined strategies are combined with an experimental validation plan.
Output: Design strategies ready for experimental validation.
Timeline: 2 weeks per sample

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High-throughput protein expression, production, and biophysical screening

High-throughput platform screening of tens to hundreds of samples in parallel, to experimentally validate protein design and engineering strategies.

Input: Protein sequences
Process:The provided protein sequences will be synthesized and cloned into expression vectors, followed by testing in bacterial expression systems. Proteins will undergo purification using an affinity-based step followed by size exclusion chromatography. The latter step, often overlooked in high-throughput workflows, is crucial for us to ensure that the protein constructs are forming monodisperse and high-quality samples suitable for structural analysis. After the expression and purification of samples in a multi-well format, we provide a biophysical readout compatible with multi-well analysis, such as protein stability or activity. This process allows us to identify which proteins—and consequently, which design strategies—are suitable for further in-depth analysis.
Output: A set of protein constructs evaluated and scored based on experimental data for expression, quality, and activity.
Timeline: 4 weeks for 384 samples.

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Grid screening with Cryo-EM

Medium-resolution grid Screening

Input: High-quality protein sample
Process: In the first step, we analyze the sample for quality and optimize CryoEM grids for high-resolution protein structure determination. Leveraging our proprietary PUXANO Grid portfolio, where PUXANO is a world leader, we ensure optimal results. Data is collected using a Talos Arctica equipped with a Falcon IVi DED. During each session, a total of 12 grids are screened, with the option to perform overnight data collection to obtain a preliminary medium-resolution 3D structure.
Output: Optimal cryoEM grid and medium-resolution protein structure
Timeline: 2 weeks per protein sample

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High-resolution protein structure determination with Cryo-EM

High-resolution protein structure determination

Input: Optimal Cryo-EM grid
Process: Once the initial analysis is complete and the ideal grid conditions have been determined, we proceed to data collection on a Titan Krios equipped with a Gatan energy filter and Falcon III/K3 camera. On average, 10,000 images are captured per session. Single particle data analysis is conducted using proprietary workflows on in-house GPU servers, ensuring rapid results. We deliver a high-resolution, sub-4-angstrom protein structure.
Output: high-resolution protein structure
Timeline: 4 weeks per sample

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Cryo-EM based nanoparticle characterization

Deep qualitative and quantitive analysis of lipid nanoparticles or other nanoparticle types using a combination of cryoEM, AI-assisted image processing with other biophysical methods.

Input: LNP samples + buffer
Process: The samples will first be optimized for cryoEM studies by adjusting the buffer and concentration using standardized procedures. Following optimization, the samples will be analyzed using our proprietary PUXANO cryoEM grid portfolio. Data acquisition will be conducted using a Talos Arctica equipped with a Falcon IVi DED. The resulting data will be analyzed using AI-assisted segmentation and analysis algorithms to determine the size distribution of the sample. Packages are available for 1, 6, or 12 samples, each with a different cost per sample. Optionally, we can include Multi-Angle Light Scattering (MALS) as an orthogonal technique to confirm the global size distribution of the sample. This addition can help strengthen conclusions and eliminate potential biases.
Output: A comprehensive report with images and statistical analysis of 1, 6, or 12 LNP samples.
Timeline: 2 weeks for the analysis of 1, 6, or 12 LNP samples.

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Applications

Vaccine Design

In silico design of protein target variations with enhanced immunogenicity followed by high throughput expression, production and qualitative analysis using protein interaction and stability tests to select a reduced set of optimized core antigen variants.