PUXANO graphene grids have become our preferred choice for a wide range of applications. They are particularly transformative for projects affected by preferred particle orientation, low sample yields, or protein disassembly. To further enhance performance for preferred particle orientation challenges, we are now fine-tuning our MultiHole Grids—featuring varied hole sizes—and developing a Tilted Grid specifically designed to mitigate orientation biases. Together, these innovations create a versatile grid portfolio, enabling the resolution of long-standing obstacles in structural determination using cryo-EM within a single experiment.